THE DIFFERENCE OF HISTOPATHOLOGICAL IMAGE OF THE WISTAR RAT’S KIDNEY ADMINISTERED WITH GRADUAL DOSAGE OF PYRETHROID

ABSTRACT

Background: Pyrethroid pesticide poisoning cases in developed and developing countries have a high incidence every year. One of the active substances in pyrethroid widely used is cypermethrin.In the 2006-2016 period, 30.789 cases of pesticide  poisoningcaused  by  cypermethrinwere  reported  in  Jiangsu  Province,  China.  Among  these  cases,  12.867 involved  men  and  17.922  involved  women.  Overall,  1.705  cases  ended  in  death. The  effectsof  cypermethrin intoxication on the kidneys, especially in humans, are little to be studied. This study aims to determine the difference in the histopathological image of the Wistarrat’s kidney.Method: The experimental research of the post-test only control group  designinvolved  24  male Wistar rats  divided  into  4  groups  randomly,  the  control  (not  given  cypermethrin), treatments of 125 mg/kgBW, 250 mg/kgBW, and 500 mg/kgBW. Cypermethrin are given orally for 14 days. After the rats wereterminated, the kidney wereprocessedto be paraffin-embedded tissues and stained with HE. Tubular injuries were  examined  using  400x  magnification  of  light  microscope  and  focused  on  closure  of  tubular  lumen  as  well  as proteinaceous  cast  inside  the  lumens. Results:The results  of  this  study  showed  that  the  means  of  histopathological damage  to  the  kidneys  increased  from  control  group  to  500  mg/kgBWtreatment  group.  Statistical  analysis  with One way  ANOVAshowed  significant  differences  (p<0.001),  continued  with Post  hoc  games  Howelltest,  there  was  a significant differences between control group with 250 mg/kgBWtreatment group and 500 mg/kgBWtreatment group, and between 125  mg/kgBWtreatment group  with 250mg/kgBWtreatment  group and 500  mg/kgBWtreatment  group. There was no significant difference between the control group with 125 mg/kgBWgroup and 250 mg/kgBWtreatment group  with  500  mg/kgBWtreatment  group.Conclusion: There  is  a  significant  difference  in  renal  histopathological  image  due  to  exposure  to  pyrethroid  (cypermethrin)  in  gradual  doses.  The  image  of  kidney  damages  can  result  in tubules  injury    which  include:  albuminous  degeneration  with  narrowing  of  the  tubular  lumen  and  hyaline  cast.  The means of tubular injury rate will increase with increasing dose of pyrethroid.Keywords:cypermethrin; kidney damage; pyrethroid

29537-65976-1-PB (1)