ABSTRACT
Background : Formaldeyde is main substance used for organ embalming and even for foods preservations. However, long and high exposure of formaldeyhde lead to various organs irritations. Honey is well-known for its natural preservative activity expected to be the soultion for the problem so there is safe alternative for organ preservations.Aim : Observation and examintaion the difference of macroscopic and microscopics feature of Wistar rats liver after immersion of formaldehyde and randu honey.Methods:Experimental with post-test only control group design using 24 male Wistar rats assample, randomly divided into 4 groups consist of: Group C (preserved with 10% formaldehyde solutions), P1 (preserved with 10% randu honey solutions), P2 (preserved with 20% randu honey solutions) and P3 (preserved with 30% randu honey solutions). The sampels were preserved for 24 hours then macroscopic (smell,color and size) and microscopic study with Hematoxylin-Eosin (HE) stain using 400x observation performed. Manja Roenigk score were used to accessed the degree of live microsopic damage.Results: There are normal cells, mild damage, moderate damage and severe damage respectively in the group C: 36%,47%,17%,0% ; P1: 0%,27%,46%,27% ; P2: 0%,77%,17%,6% ; P3: 47%,33%,17%,3%. Kruskal-Wallis test showed significant results with p=0.000. With Mann-Whitney test, significant results were found between group C with P1 (0.000), C with P2 (0.006), P1 with P2 (0.000), P1 with P3 (0.000) and P2 with P3 (0.003). However, test result were not significant berween group C with P3 (0.684).Conclusions: There were differences in macroscopics and microscopic features between Wistar rats liver after immersion in formaldehyde and randu honey.The best features showed in samples preserved in 30% Randu Honey solutions.Keyword: formaldehyde, honey, embalming, organ preservation